A bacterial strain was isolated from the oil-contaminated soil by selective enrichment on n-hexadecane as the sole carbon source.Based on the morphological observation and automatic identification,the strain was preliminarily identified to be genus Pseudomonas aeruginosa.The ability for the isolated strain to biodegrade n-hexadecane was evaluated by determining n-hexadecane concentration of the inoculated culture medium on a gas chromatography.Results showed that the isolated strain was markedly effective in biodegrading n-hexadecane.Furthermore,the ability for the isolated strain to biodegrade n-hexadecane was the best at the pH value of 7.0.