Objective To investigate An easy and accurate method for the identification of Gardenia jasminoides Ellis and Gardenia jasminoides Ellis var.grandiflora Nakai.Method The protein polypeptide was analysed in Gardenia jasminoides Ellis and Gardenia jasminoides Ellis var.grandiflora Nakai respectively by high-performance capillary electrophoresis(HPCE).Result The HPCE result showed that the protein polypeptide of Gardenia jasminoides Ellis was significantly different from that of Gardenia jasminoides Ellis var.grandiflora Nakai with a good repeatability.Conclusion HPCE can be used for the identification of Gardenia jasminoides Ellis from Gardenia jasminoides Ellis var.grandiflora Nakai.