Qoning and sequence analysis of NSP4 and NSPS genes of a novel rotavirus strain J19

To clone and sequence NSP4 and NSP5 genes of a novel rotavirus strain J19 causing adult diarrhea. Methods NSP4 and NSP5 genes of J19 were amplified by an improved single primer sequence-independent amplification method, cloned into pMD18-T vector, and then sequenced. Ammo acid sequence comparison and phylogenetic analysis of NSP4 and NSP5 of J19 were made with the known proteins of other rotaviruses. Results Genomic segment 10,11 of J19 encoding NSP4 and NSP5 were 739 bp and 649 bp in length, and the deduced proteins were 213 AA and 176 AA respectively. NSP4 and NSP5 of J19 showed moderately higher identities to group B adult diarrhea rotavirus strain Bang373(20. 3%) and group B bovine rotavirus strain db101(29.5%)respectively. Phylogenetic analysis of NSP4 and NSP5 of J19 showed that J19 was positioned close to the ancestral nodes of group A, B, and C lineages. Conclusion NSP4 and NSP5 of J19 differed significantly with those of any other known rotaviruses. Protein sequence analysis and phylogenetic analysis of J19 showed that the novel rotavirus and the classic may originate from a common ancestral virus.