In this paper, the laccase activities of 18 woodrot fungi were studied. The results showed that the maxmum activity of laccase of Pleurotus lignetilis was 605.0 u·mL-1, then the maxmum activity of laccase of Trametes trogii and Corilus versicolar were 440.0 u·mL-1 and 400.0 u·mL-1. Different pH values and inorganic ions had different effects on laccase activities of Pleurotus lignetilis. The optimum pH value of laccase of Pleurotus lignetilis was 4.6, Cu2+ ?Mg2+?Na+?Co2+ increased laccase activities whereas Ag+?K+?Ca2+?Hg2+?Fe2+ ?Cl- and Zn2+ significantly inhibited it.