Isolation,identification and enzyme characteristics of a strain producing chitosanase

The aim of this paper was to screen chitosanase producing strain and to investigate the characteristics of the chitosanase produced by this strain.Chitsoan was used as the sole carbon source for the acclimatization and screening of strain.A strain with a higher chitosanase activity was isolated from soil.It was identified as Aspergillus fumigatus by its morphology in solid medium and by analyzing the sequence of ITS.The optimum temperature and pH were 55℃ and 5.6,respectively.And the chitosanase is stable at 45~60℃ and pH 4.0~8.0,respectively.The activity of the enzyme is strongly promoted by Mn 2+ and inhibited by Ag +,Cu 2 + and Zn 2 +.The properties of chitosanase indicated that it has a great potential application in chitosan degradation.