Protective Effect of Huganning Tablet on CCl_4 -Induced Liver Cell Injury in vivo and in vitro

Objective:To explore the protective effect of Huganning tablet on the CCl4-induced hepatocyte injury animal models.Methods:The cultured normal L-O2 hepatocytes and the carbon tetrachloride(CCl4)-induced hepatocytes for 12 h in vitro were divided into normal control group,model control group and Huganning solution group.For the second 12 h,MTT assay was used to detect the cell viability in each group.At the same time,the model of injury hepatocytes were divided into CCl4 model group,blank serum group,Huganning-containing serum group,and MTT assay was used to detect the cell viability in each group after the third 12 h.In addition,36 SD rats were evenly divided into three groups:normal control group,model control group,Huganning solution group.Each group was given appropriate liquid via gavage once a day for consecutive 7 days.Six hours after the last administration,the rats of model control group and Huganning solution group received intraperitoneal injection of 50% CCl4 peanut oil(2 mL/kg) in order to form liver injury model.Serum ALT and AST of all the rats in each group were detected after 24 h.Results:The OD values of CCl4 model group were lower than that of normal control group(P 0.01),while that of Huganning solution group was significantly higher than that of CCl4 model group but lower than that of normal serum group(P 0.05).The OD values of Huganning-containing serum group were significantly higher than that of CCl4 model group and blank serum group(P 0.05).The rats' serum ALT and AST levels of model control group and Huganning solution group were higher than those of normal control group(P 0.01).However,the rats' serum ALT and AST levels of Huganning solution group were lower than that of model control group(P 0.05).Conclusion:Huganning Tablet had effect of anti-liver cell damage and protecting liver cells.