材料科学
骨愈合
骨桥蛋白
碱性磷酸酶
生物医学工程
体内
固定(群体遗传学)
股骨骨折
骨折
骨矿物
股骨
化学
外科
骨质疏松症
医学
病理
生物
内科学
生物化学
生物技术
放射科
基因
酶
作者
Pei Han,Chong Peng,Shaoxiang Zhang,Changli Zhao,Jiahua Ni,Yuanzhuang Zhang,Wanrun Zhong,Peng Hou,Xiaonong Zhang,Yufeng Zheng,Yimin Chai
出处
期刊:Biomaterials
[Elsevier]
日期:2015-09-01
卷期号:64: 57-69
被引量:192
标识
DOI:10.1016/j.biomaterials.2015.06.031
摘要
High-purity magnesium (HP Mg) takes advantage in no alloying toxic elements and slower degradation rate in lack of second phases and micro-galvanic corrosion. In this study, as rolled HP Mg was fabricated into screws and went through in vitro immersion tests, cytotoxicity test and bioactive analysis. The HP Mg screws performed uniform corrosion behavior in vitro, and its extraction promoted cell viability, bone alkaline phosphatase (ALP) activity, and mRNA expression of osteogenic differentiation related gene, i.e. ALP, osteopontin (OPN) and RUNX2 of human bone marrow mesenchymal stem cells (hBMSCs). Then HP Mg screws were implanted in vivo as load-bearing implant to fix bone fracture and subsequently gross observation, range of motion (ROM), X-ray scanning, qualitative micro-computed tomography (μCT) analysis, histological analysis, bending-force test and SEM morphology of retrieved screws were performed respectively at 4, 8, 16 and 24 weeks. As a result, the retrieved HP Mg screws in fixation of rabbit femoral intracondylar fracture showed uniform degradation morphology and enough bending force. However, part of PLLA screws was broken in bolt, although its screw thread was still intact. Good osseointegration was revealed surrounding HP Mg screws and increased bone volume and bone mineral density were detected at fracture gap, indicating the rigid fixation and enhanced fracture healing process provided by HP Mg screws. Consequently, the HP Mg showed great potential as internal fixation devices in intra-articular fracture operation.
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