Effect of Sudden Salt Stress on Ion Fluxes in Intact Wheat Suspension Cells

Although salinity is one of the major problems limiting agricultural production around the world, the underlying mechanisms of high NaCl perception and tolerance are still poorly understood. The effects of different bathing solutions and fusicoccin (FC), a known activator of plasma membrane ATPase, on plasma membrane potential ( Em ) and net fluxes of Na + , K + and H + were studied in wheat suspension cells ( Triticum aestivum ) in response to different NaCl treatments. Em of cells in Murashige and Skoog (MS) medium was less negative than in cells exposed to a medium containing 10 m M KCl + 0.1 m M CaCl 2 (KSM) and to a basic salt medium (BSM), containing 1 m M KCl and 0.1 m M CaCl 2 . Multiphasic Na + accumulation in cells was observed, peaking at 13 min after addition of 120 m M NaCl to MS medium. This time scale was in good agreement with net Na + flux changes measured non-invasively by moving ion-selective microelectrodes (the MIFE system). When 120 m M NaCl was added to all media studied, a quick rise of Na + influx was reversed within the first 20 min. In both 120 and 20 m M NaCl treatments in MS medium, net Na + efflux was observed, indicating that active Na + transporters function in the plant cell response to salt stress. Lower external K + concentrations (KSM and BSM) and FC pre-treatment caused shifts in Na + fluxes towards net influx at 120 m M NaCl stress. Copyright 2000 Annals of Botany Company