IL-4 and CD40 ligation affect differently the differentiation, maturation, and function of human CD34+ cell-derived CD1a+CD14− and CD1a−CD14+ dendritic cell precursors in vitro

生物 CD14型 树突状细胞 细胞生物学 功能(生物学) 体外 细胞分化 CD40 免疫学 免疫系统 生物化学 基因 细胞毒性T细胞
作者
Bruno Canque,Sandrine Camus,Micaël Yagello,Jean Claude Gluckman
出处
期刊:Journal of Leukocyte Biology [Oxford University Press]
卷期号:64 (2): 235-244 被引量:48
标识
DOI:10.1002/jlb.64.2.235
摘要

Abstract We examined the effect of interleukin (IL)-4 or CD40 ligation on the differentiation and maturation of CD1a+CD14– and CD1a–CD14+ dendritic cell (DC) precursors. Cord blood CD34+ cells were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor α (TNF-α), to which stem cell factor and Flt-3 ligand were added for 5 days. Phenotypic analysis of DC precursors on culture day 7 showed that CD1a+CD14– cells expressed higher CD11c and CD80 levels and lower CD116/GM-CSFR and CCR-5 levels than their CD1a–CD14+ counterparts. Culturing CD1a+CD14– precursors with GM-CSF and TNF-α resulted in DC with heterogeneous CD1a, HLA;SMDR (DR), CD11b, and CD83 expression, 10% of which acquired CD14. IL-4 and CD40 ligation affected their differentiation in contrasting ways: IL-4 induced CD1ahiCD14– DRloCD11b+CD83–S100+ DC with reduced MLR-stimulating capacity, whereas CD40 ligation led to CD1alo/-CD14–CD40–DRhiCD11b–CD83+S100+/– DC with stronger MLR-stimulating capacity. Also, both IL-4 and CD40 ligation promoted RelB expression and nuclear translocation. When CD1a–CD14+ precursors were maintained in only the presence of GM-CSF and TNF-α, this led to mixed populations of adherent macrophages and nonadherent CD1a–CD14+ monocytes, and of CD1a+CD14– and CD1a+CD14+ DC, which were DRloCD11b+-CD83–S100–. IL-4 or CD40 ligation prevented their differentiation into macrophages and resulted in DC with phenotypes close to those issued from CD1a+CD14– precursors, with only a minority staying CD14+ but most being S100–; their MLR-stimulating capacity also increased but remained lower than that of DC differentiated from CD1a+CD14– precursors. Thus, IL-4 or CD40 ligation induced CD1a+CD14– and CD1a–CD14+ DC precursors to differentiate into phenotypically close but functionally different DC populations, suggesting that DC function is primarily determined by their origin. The heterogeneity of DC should then be related to different developmental pathways and to different stages of maturation/activation. J. Leukoc. Biol. 64: 235–244; 1998.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
h w wang完成签到,获得积分10
4秒前
Dream完成签到,获得积分0
5秒前
Anna完成签到 ,获得积分10
5秒前
5秒前
点点完成签到 ,获得积分10
6秒前
Hunter完成签到,获得积分10
6秒前
lydiaabc完成签到,获得积分10
11秒前
15秒前
求知小生完成签到,获得积分10
16秒前
21秒前
25秒前
梨里完成签到,获得积分10
30秒前
冯不言发布了新的文献求助10
31秒前
31秒前
啊福完成签到,获得积分10
33秒前
徐彬荣完成签到,获得积分10
33秒前
青檬完成签到 ,获得积分10
35秒前
36秒前
36秒前
量子星尘发布了新的文献求助10
37秒前
39秒前
哈哈哈完成签到,获得积分10
42秒前
42秒前
Balance Man完成签到 ,获得积分0
43秒前
mix完成签到 ,获得积分10
50秒前
小白完成签到,获得积分10
51秒前
瘦瘦的迎梦完成签到 ,获得积分10
52秒前
wwrjj完成签到,获得积分10
52秒前
赵西里完成签到,获得积分10
56秒前
11完成签到 ,获得积分10
57秒前
小公牛完成签到 ,获得积分10
57秒前
57秒前
1分钟前
西洲完成签到 ,获得积分10
1分钟前
1分钟前
xiaofeiyan完成签到 ,获得积分10
1分钟前
笑点低蜜蜂完成签到 ,获得积分10
1分钟前
研学弟完成签到,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Social Research Methods (4th Edition) by Maggie Walter (2019) 2390
A new approach to the extrapolation of accelerated life test data 1000
北师大毕业论文 基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 390
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4008763
求助须知:如何正确求助?哪些是违规求助? 3548409
关于积分的说明 11298823
捐赠科研通 3283064
什么是DOI,文献DOI怎么找? 1810290
邀请新用户注册赠送积分活动 886000
科研通“疑难数据库(出版商)”最低求助积分说明 811220