口蹄疫病毒
抗原
病毒学
血清型
口蹄疫
效力
抗体
接种疫苗
金标准(测试)
医学
口疮病毒
病毒
免疫学
生物
体外
内科学
生物化学
作者
Xia Feng,Jingjiao Ma,Shiqi Sun,Huichen Guo,Youyou Yang,Ye Jin,Gengxu Zhou,Jijun He,Jianpeng Guo,Shuhui Qi,Min Lin,Hu Cai,Xiangtao Liu
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2016-03-01
卷期号:11 (3): e0149569-e0149569
被引量:18
标识
DOI:10.1371/journal.pone.0149569
摘要
The efficacy of an inactivated foot-and-mouth disease (FMD) vaccine is mainly dependent on the integrity of the foot-and-mouth disease virus (FMDV) particles. At present, the standard method to quantify the active component, the 146S antigen, of FMD vaccines is sucrose density gradient (SDG) analysis. However, this method is highly operator dependent and difficult to automate. In contrast, the enzyme-linked immunosorbent assay (ELISA) is a time-saving technique that provides greater simplicity and sensitivity. To establish a valid method to detect and quantify the 146S antigen of a serotype O FMD vaccine, a double-antibody sandwich (DAS) ELISA was compared with an SDG analysis. The DAS ELISA was highly correlated with the SDG method (R2 = 0.9215, P<0.01). In contrast to the SDG method, the DAS ELISA was rapid, robust, repeatable and highly sensitive, with a minimum quantification limit of 0.06 μg/mL. This method can be used to determine the effective antigen yields in inactivated vaccines and thus represents an alternative for assessing the potency of FMD vaccines in vitro. But it still needs to be prospectively validated by analyzing a new vaccine preparation and determining the proper protective dose followed by an in vivo vaccination-challenge study to confirm the ELISA findings.
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