Transmission Electron Microscopy of the Peripheral Nervous System: Methods, Sample Preparation, and Case Studies

Recommendations and practical considerations for sample collection, fixation, preparation and imaging of peripheral nerves and ganglia for resin embedding, light microscopic evaluation and transmission electron microscopy for ultrastructural pathology evaluation, based on the experience of the authors. Uses and limitations of transmission election microscopy in nonclinical safety studies is demonstrated through case studies of control and test article-dosed nonclinical material to highlight common artifacts and findings in this increasingly important tissue for emerging pharmaceutical modalities.