Piriformospora indica colonization enhances remediation of cadmium and chromium co-contaminated soils by king grass through plant growth promotion and rhizosphere microecological regulation

Poor plant growth and low pollutant bioavailability in contaminated soils limit phytoremediation efficiency. Pot experiments were conducted to investigate the effects and mechanisms of Piriformospora indica inoculation on the phytoremediation of Cd-Cr co-contaminated soils from farmland using king grass. P. indica colonization increased plant biomass by 20.4-24.6% and enhanced Cd/Cr accumulation in root, stem and leave tissues. Root vascular cylinder and cortex were the major structures for Cd/Cr transportation in plants. The amounts of Cd and Cr extracted by king grass considerably increased in the presence of P. indica (by 31.5-88.9% and 22.4-38.4%, respectively), as did the removal efficiency of both metals from soils (by 13.2-32.2% and 23.2-33.5%, respectively). Cd/Cr phytoextraction was closely related to the contents of alkanes, lipids and acids in root exudates. Following inoculation, the respiration of microbial sulfur compounds was promoted in soils at low and medium pollution levels, whereas nitrogen fixation and nitrification were reduced at high pollution level. This study demonstrates that P. indica inoculation enhances the phytoremediation efficiency of king grass for Cd-Cr co-contaminated soils through multiple regulation of plant growth, rhizosphere environment, root exudation and soil microbial function.