Available Strategies for Improving the Biosynthesis of Methionine: A Review

Methionine is the only nonpolar α-amino acid containing sulfur among the eight essential amino acids and is closely related to the metabolism of sulfur-containing compounds in the human body. Widely used in feed, medicine, food, and other fields, the market demand is increasing annually. However, low productivity and high cost largely limit the industrial production of methionine, and many novel production methods still have their own disadvantages. In this paper, the available methods for synthesizing methionine are reviewed and discussed. The latest strategies for improving methionine production are further introduced, including culture medium optimization, mutation technology, expression of key genes in the metabolic pathway, knockout and recombination, as well as the engineering of membrane transporters, the fermentation-enzymatic coupling route, and innovation of CO