固氮酶
变色固氮菌
固氮
细菌
紫色细菌
化学
光合作用
材料科学
氮气
生物化学
有机化学
生物
光合反应中心
遗传学
作者
Yicheng Zeng,Haotian Bai,Wen Yu,Shengpeng Xia,Qi Shen,Yiming Huang,Fengting Lv,Guillermo C. Bazan,Shu Wang
标识
DOI:10.1002/anie.202303877
摘要
Abstract A conductive polymer‐based photosynthetic biohybrid is constructed to enhance biological nitrogen fixation by increasing nitrogenase activity in the non‐photosynthetic bacterium Azotobacter Chroococcum ( A. Chroococcum ). The light‐harvesting cationic poly(fluorene‐alt‐phenylene) (PFP) electrostatically binds to the surface of the bacteria and possesses satisfactory conductivity to facilitate electron transfer to the bacterium, promoting the nitrogen fixation pathway through redox proteins on the surface of the bacteria when under illumination. Therefore, the nitrogenase activity, hydrogen, NH 4 + ‐N and L‐amino acids production are increased by 260 %, 37 %, 44 %, and 47 %, respectively. The expression levels of nif D and nif K encoding molybdenum‐iron (MoFe) protein and relevant nitrogen‐fixing proteins are up‐regulated. These photoactive conductive polymer‐bacteria biohybrids provide a new method for improving the biological nitrogen fixation capability of non‐photosynthetic nitrogen‐fixing bacteria.
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