化学
电化学发光
石墨氮化碳
检出限
生物传感器
空位缺陷
碳纤维
氮化碳
纳米技术
有机化学
色谱法
材料科学
结晶学
催化作用
生物化学
光催化
复合数
复合材料
作者
Linlei Liu,Yong Zhu,Haijun Wang,Yue Zhang,Yaqin Chai,Ruo Yuan
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-08-29
卷期号:94 (36): 12444-12451
被引量:27
标识
DOI:10.1021/acs.analchem.2c02462
摘要
Herein, a supersensitive biosensor was constructed by using graphitic carbon nitride with a carbon vacancy (VC-g-C3N4) as an efficient electrochemiluminescence (ECL) emitter for detection of microRNA-21 (miRNA-21). Impressively, VC-g-C3N4 could be prepared by formaldehyde (HCHO)-assisted urea ploycondensation, and the concentration of the carbon vacancy could be controlled by adjusting the dosage of HCHO to improve the ECL performance, in which the carbon vacancy could improve the charge carrier transfer to enhance the conductivity and it also could be used as an electron trap to prevent electrode passivation and facilitate the adsorption of coreactant S2O82- to accelerate its reduction. Compared with original g-C3N4, the introduction of carbon vacancies resulted in a significant enhancement of the ECL efficiency of VC-g-C3N4. With the aid of improved cascade strand displacement amplification (IC-SDA), the ECL biosensor realized sensitive detection of miRNA-21 with a low detection limit of 3.34 aM. This successful strategy promoted the development of g-C3N4 in the ECL field to construct the sensitive biosensor for molecular and disease diagnoses.
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