谷胱甘肽
化学
半胱氨酸
硫化氢
荧光
乙酰半胱氨酸
硫醇
香豆素
高效液相色谱法
组合化学
生物活性
生物化学
硫黄
色谱法
抗氧化剂
体外
有机化学
酶
物理
量子力学
作者
Daniel Słowiński,Małgorzata Świerczyńska,Jarosław Romański,Radosław Podsiadły
出处
期刊:Molecules
[MDPI AG]
日期:2022-11-28
卷期号:27 (23): 8305-8305
被引量:5
标识
DOI:10.3390/molecules27238305
摘要
Hydrogen sulfide (H2S) and its bioderivatives analogs, such as L-cysteine (L-Cys) and glutathione (GSH), are ubiquitous biological thiols in the physiological and pathological processes of living systems. Their aberrant concentration levels are associated with many diseases. Although several NBD-based fluorescence probes have been developed to detect biological thiols, the HPLC-detection of H2S, GSH, L-Cys, and N-acetylcysteine-specific products has not been described. Herein, a novel NBD-derived pro-coumarin probe has been synthesized and used to develop a new strategy for the triple mode detection of H2S and such thiols as GSH, L-Cys, and NAC. Hydrogen sulfide and those biothiols at physiological pH release fluorescent coumarin from the probe and cause a significant fluorescence enhancement at 473 nm. The appropriate NBD-derived product for H2S, L-Cys, GSH, and NAC has a different color and retention time that allows distinguishing these biological thiols meaning the probe has a great possibility in the biological application. Fluorescent imaging combined with colorimetric and HPLC detection of H2S/biothiol-specific product(s) brings a potential tool for confirming the presence of biological thiols and determining concentrations in various aqueous biological samples.
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