Immobilized short-chain dehydrogenase/reductase on Fe3O4 particles acts as a magnetically recoverable biocatalyst component in patulin bio-detoxification system

展青霉素 化学 戒毒(替代医学) 固定化酶 辅因子 生物催化 色谱法 烟酰胺腺嘌呤二核苷酸 烟酰胺腺嘌呤二核苷酸磷酸 醇脱氢酶 真菌毒素 生物化学 食品科学 NAD+激酶 催化作用 医学 离子液体 替代医学 病理 氧化酶试验
作者
Mengyang Xing,Yong Chen,Wanqin Dai,Xiao He,Boqiang Li,Shiping Tian
出处
期刊:Journal of Hazardous Materials [Elsevier BV]
卷期号:448: 130986-130986 被引量:31
标识
DOI:10.1016/j.jhazmat.2023.130986
摘要

Patulin is one of the most important mycotoxins that contaminates fruit-derived products and causes acute or chronic toxicity in humans. In the present study, a novel patulin-degrading enzyme preparation was developed by taking a short-chain dehydrogenase/reductase and covalently linking it to dopamine/polyethyleneimine co-deposited magnetic Fe3O4 particles. Optimum immobilization provided 63% immobilization efficiency and 62% activity recovery. Moreover, the immobilization protocol substantially improved thermal and storage stabilities, proteolysis resistance, and reusability. Using reduced nicotinamide adenine dinucleotide phosphate as a cofactor, the immobilized enzyme exhibited a detoxification rate of 100% in phosphate-buffered saline and a detoxification rate of more than 80% in apple juice. The immobilized enzyme did not cause adverse effects on juice quality and could be magnetically separated quickly after detoxification to ensure convenient recycling. Moreover, it did not exhibit cytotoxicity against a human gastric mucosal epithelial cell line at a concentration of 100 mg/L. Consequently, the immobilized enzyme as a biocatalyst had the characteristics of high efficiency, stability, safety, and easy separation, establishing the first step in building a bio-detoxification system to control patulin contamination in juice and beverage products.
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