线粒体DNA
生物
基因组编辑
DNA
Cas9
点突变
胞嘧啶
碱基对
RNA编辑
遗传学
胞嘧啶脱氨酶
清脆的
计算生物学
分子生物学
基因
突变
核糖核酸
遗传增强
作者
Zongyi Yi,Xiaoxue Zhang,Wei Tang,Ying Yu,Xiaoxu Wei,Xue Zhang,Wensheng Wei
标识
DOI:10.1038/s41587-023-01791-y
摘要
A number of mitochondrial diseases in humans are caused by point mutations that could be corrected by base editors, but delivery of CRISPR guide RNAs into the mitochondria is difficult. In this study, we present mitochondrial DNA base editors (mitoBEs), which combine a transcription activator-like effector (TALE)-fused nickase and a deaminase for precise base editing in mitochondrial DNA. Combining mitochondria-localized, programmable TALE binding proteins with the nickase MutH or Nt.BspD6I(C) and either the single-stranded DNA-specific adenine deaminase TadA8e or the cytosine deaminase ABOBEC1 and UGI, we achieve A-to-G or C-to-T base editing with up to 77% efficiency and high specificity. We find that mitoBEs are DNA strand-selective mitochondrial base editors, with editing results more likely to be retained on the nonnicked DNA strand. Furthermore, we correct pathogenic mitochondrial DNA mutations in patient-derived cells by delivering mitoBEs encoded in circular RNAs. mitoBEs offer a precise, efficient DNA editing tool with broad applicability for therapy in mitochondrial genetic diseases.
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