内质网
未折叠蛋白反应
荧光
发色团
自噬
绿色荧光蛋白
细胞内
化学
粘度
细胞生物学
平衡
生物物理学
生物化学
生物
材料科学
细胞凋亡
光化学
物理
基因
复合材料
量子力学
作者
Xiaoqin Wei,Yiming Zhu,Yu Xiang,Lei Cai,Nanan Ruan,Linjing Wu,Nengqin Jia,Tony D. James,Chusen Huang
摘要
The occurrence of endoplasmic reticulum (ER) stress is the main cause of a variety of biological processes that are closely related to numerous diseases. The homeostasis of the ER microenvironment can be disrupted under ER stress. In this research, by linking a pentafluorophenyl to the green fluorescent protein chromophore, we have developed a new ER-targeting fluorescent probe (GE-Y) for measuring changes of intracellular ER viscosity caused by ER stress. Importantly, an increase in ER viscosity was observed using GE-Y in cells undergoing autophagy. As such, our research provides an ideal tool for studying ER stress and autophagy.
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