污染
痤疮丙酸杆菌
生物反应器
细菌
微生物学
生化工程
生物
化学
环境科学
生态学
遗传学
植物
工程类
出处
期刊:Pda Journal of Pharmaceutical Science and Technology
[Parenteral Drug Association, Inc.]
日期:2019-12-16
卷期号:74 (5): 592-601
被引量:1
标识
DOI:10.5731/pdajpst.2019.010710
摘要
Mammalian cell lines constitute an important part in the manufacture of therapeutic proteins. A contamination-free operation of a mammalian cell bioreactor demands constant attention to details. Cutibacterium acnes, a slow-growing bacterium, is a common skin flora and is often associated with mammalian cell bioreactor contaminations. This paper reviews the literature published about such C. acnes contaminations and presents three hypothetical contamination scenarios based on the author’s experience in the hope of fostering early detection of contamination events. Bioreactor process parameters such as unusual fluctuation in dissolved oxygen, high levels of ammonia, and microscopic examination have been identified as useful tools to detect slow-growing bacteria contamination.
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