Three-dimensional bioprinted hepatorganoids in liver failure

We read with interest the recent study by Yang et al , who reported the construction of three-dimensional bioprinted hepatorganoids (3DP-HOs) using HepaRG cells and bioinks.1 We congratulate the authors for this pioneering study that demonstrates the fabrication and in vivo application of functional 3DP-HOs in mice models of liver failure. The study would pave the way for the use of three-dimensional (3D) bioprinted primary hepatocytes and/or hepatic progenitor cells as possible regenerative therapies in liver diseases. However, there are some caveats in the study, which need to be highlighted. First, the study showed that after 7 days of differentiation in vitro, HepaRG cells in 3DP-HOs expressed hepatic marker genes and exhibited albumin secretion (figure 2). However, it is to be noted that most of …