乙型肝炎表面抗原
HBeAg
分子生物学
乙型肝炎病毒
免疫印迹
病毒复制
抄写(语言学)
病毒学
核糖核酸
转染
乙型肝炎病毒β前体
基因表达
基因
生物
医学
病毒
乙型肝炎病毒DNA聚合酶
生物化学
哲学
语言学
作者
Juan Li,Fan-Wei Liu,Dongbo Wu,En‐Qiang Chen,Xiangjun Chen,Shouchun Chen,Cong Liu,Lian-Shan Zhao,Hong Tang,Tong Zhou
标识
DOI:10.1016/j.ajg.2020.05.002
摘要
To investigate the role of low-concentration TRAIL on HBV replication and expression. MTT assay was performed to determine the minimum concentrations of TRAIL protein in HepG2 cell apoptosis. HepG2 cells were transfected by HBV replication plasmid pHBV4.1. After the treatment with low concentration of TRAIL, the culture supernatant was collected to detect HBsAg and HBeAg by ELISA. Proteins were extracted from the resulted cells, followed by total RNA and HBV DNA intermediate replication. Southern Blot and Northern Blot were carried out to detect HBV RNA and HBV DNA replication intermediates, respectively. RT-PCR and Western Blot were carried out to detect gene and protein expressions for HNF4α, PPARα, and RXRα, respectively. 50 ng/ml of TRAIL protein led to significant decline on the secretions of HBsAg and HBeAg. Expression levels of HBV RNA and HBV DNA replication intermediates were significantly decreased too. In addition, gene and protein expressions of HNF4α, PPARα and RXRα also dropped, especially for PPARα whose expressions significantly decreased. TRAIL could inhibit HBV replication and expression by downregulating the expressions of liver-enriched transcription factors HNF4α, PPARα, and RXRα.
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