适体
金黄色葡萄球菌
费斯特共振能量转移
化学
检出限
橙汁
替考拉宁
胶体金
纳米颗粒
量子点
线性范围
纳米技术
荧光
色谱法
细菌
分子生物学
材料科学
生物
食品科学
物理
量子力学
遗传学
万古霉素
作者
Xiaoqi Tao,Ziyi Liao,Yaqing Zhang,Fei Fu,Mengqi Hao,Yang Song,Erqun Song
标识
DOI:10.1016/j.cclet.2020.07.020
摘要
The detection of bacterial pathogen such as Staphylococcus aureus (S. aureus) is essential for the regulation of food hygiene and disease diagnosis. Herein, we developed a simple one-step fluorescence resonance energy transfer (FRET)-based sensor for specific and sensitive detection of S. aureus in food and serum samples, in which aptamer-modified quantum dots (aptamer-QDs) was employed as the energy donor and antibiotic of teicoplanin functionalized-gold nanoparticles (Teico-AuNPs) was chosen as the energy acceptor. Within 1 h, the FRET-based sensor showed a linear range of from 10 cfu/mL to 5 × 108 cfu/mL, with the low limit of detection (LOD, 2 cfu/mL) for S. aureus in buffer. When further applied to assay S. aureus in real samples, the FRET-based sensor showed good recoveries ranging from 84.5% to 110.0%, with relative standard derivations (RSDs) of 0.01%–0.44% and a LOD of 100 cfu/mL in milk, orange juice and human serum.
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