色谱法
化学
氧化苦参碱
质谱法
检出限
串联质谱法
人参
根(腹足类)
苦参碱
液相色谱-质谱法
黄芪甲苷
高效液相色谱法
医学
生物
病理
替代医学
植物
作者
Mengmeng Jia,Beibei Zhang,Yinliang Qi,Jing Yang,Zhihong Yao,Z. H. Qin,Xiaojian Zhang,Xin‐Sheng Yao
标识
DOI:10.1002/jssc.201900878
摘要
Abstract Kang‐Ai injection, which is composed of Astragali Radix , Ginseng Radix et Rhizoma , and kushenin , is extensively used in China as an adjuvant therapy for many types of cancer and chronic hepatitis B. In the present study, 47 herbal compounds (11 alkaloids, 8 astragalosides, and 28 ginsenosides), were detected in Kang‐Ai injection by ultra‐high‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry, of which 31 were identified using authentic standards. Additionally, a practical ultra‐high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry method was employed for simultaneous quantitative detection (31 available compounds), and relative quantitative detection (16 unavailable compounds) within 10 min. The limit of detection and limit of quantification was 0.11‐2.22 and 0.53‐11.08 ng/mL, respectively. Altogether, content levels of each compound ranged from 0.03 to 9835.57 μg/mL. Furthermore, chemometric analysis indicated oxymatrine, astragaloside IV, ginsenosides Rg1 and Re, and matrine had the greatest effect on concentration fluctuation. Therefore, we suggested these five compounds should be monitored during the manufacturing process. This method can be applied to provide crucial chemical profiles and quality assessments for Kang‐Ai injection, guaranteeing the safety, effectiveness, and controllability of the drug in clinics.
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