Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation

材料科学 压电 脚手架 生物医学工程 血管生成 组织工程 体内 复合材料 医学 生物 癌症研究 生物技术
作者
Wenwen Liu,Xiaokang Li,Yilai Jiao,Cong Wu,Shuo Guo,Xin Xiao,Xinghui Wei,Jie Wu,Peng Gao,Ning Wang,Yajie Lu,Zhen Tang,Quanming Zhao,Jinsong Zhang,Yufei Tang,Lei Shi,Zheng Guo
出处
期刊:ACS Applied Materials & Interfaces [American Chemical Society]
卷期号:12 (46): 51885-51903 被引量:80
标识
DOI:10.1021/acsami.0c10957
摘要

Bone defect repair at load-bearing sites is a challenging clinical problem for orthopedists. Defect reconstruction with implants is the most common treatment; however, it requires the implant to have good mechanical properties and the capacity to promote bone formation. In recent years, the piezoelectric effect, in which electrical activity can be generated due to mechanical deformation, of native bone, which promotes bone formation, has been increasingly valued. Therefore, implants with piezoelectric effects have also attracted great attention from orthopedists. In this study, we developed a bioactive composite scaffold consisting of BaTiO3, a piezoelectric ceramic material, coated on porous Ti6Al4V. This composite scaffold showed not only appropriate mechanical properties, sufficient bone and blood vessel ingrowth space, and a suitable material surface topography but also a reconstructed electromagnetic microenvironment. The osteoconductive and osteoinductive properties of the scaffold were reflected by the proliferation, migration, and osteogenic differentiation of mesenchymal stem cells. The ability of the scaffold to support vascularization was reflected by the proliferation and migration of human umbilical vein endothelial cells and their secretion of VEGF and PDGF-BB. A well-established sheep spinal fusion model was used to evaluate bony fusion in vivo. Sheep underwent implantation with different scaffolds, and X-ray, micro-computed tomography, van Gieson staining, and elemental energy-dispersive spectroscopy were used to analyze bone formation. Isolated cervical angiography and visualization analysis were used to assess angiogenesis at 4 and 8 months after transplantation. The results of cellular and animal studies showed that the piezoelectric effect could significantly reinforce osteogenesis and angiogenesis. Furthermore, we also discuss the molecular mechanism by which the piezoelectric effect promotes osteogenic differentiation and vascularization. In summary, Ti6Al4V scaffold coated with BaTiO3 is a promising composite biomaterial for repairing bone defects, especially at load-bearing sites, that may have great clinical translation potential.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
wuwei完成签到,获得积分10
刚刚
刚刚
Yurole完成签到,获得积分10
1秒前
Aowu应助jack采纳,获得10
1秒前
3秒前
memebao完成签到,获得积分10
3秒前
3秒前
无限一凤完成签到 ,获得积分10
3秒前
忐忑的老虎完成签到,获得积分10
4秒前
Gaojin锦完成签到,获得积分10
4秒前
陈帅完成签到,获得积分20
6秒前
6秒前
YYYYWZ驳回了慕青应助
7秒前
科研通AI5应助SZY采纳,获得10
8秒前
小超人发布了新的文献求助10
8秒前
8秒前
叮叮叮完成签到,获得积分10
10秒前
斯文败类应助Rjy采纳,获得10
10秒前
11秒前
12秒前
12秒前
852应助科研通管家采纳,获得10
12秒前
酷波er应助科研通管家采纳,获得10
12秒前
12秒前
宝玉发布了新的文献求助10
13秒前
13秒前
科研通AI5应助super采纳,获得30
13秒前
轵关宣方完成签到,获得积分10
14秒前
清秀寇发布了新的文献求助10
14秒前
15秒前
boob完成签到,获得积分10
15秒前
嚭嚭发布了新的文献求助10
15秒前
William完成签到 ,获得积分20
15秒前
叶楠关注了科研通微信公众号
17秒前
ZCY发布了新的文献求助80
17秒前
正统发布了新的文献求助10
18秒前
18秒前
大模型应助鄢廷芮采纳,获得10
18秒前
yy完成签到,获得积分10
19秒前
19秒前
高分求助中
Les Mantodea de Guyane Insecta, Polyneoptera 2500
One Man Talking: Selected Essays of Shao Xunmei, 1929–1939 (PDF!) 1000
Technologies supporting mass customization of apparel: A pilot project 450
A Field Guide to the Amphibians and Reptiles of Madagascar - Frank Glaw and Miguel Vences - 3rd Edition 400
China Gadabouts: New Frontiers of Humanitarian Nursing, 1941–51 400
The Healthy Socialist Life in Maoist China, 1949–1980 400
Walking a Tightrope: Memories of Wu Jieping, Personal Physician to China's Leaders 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3787319
求助须知:如何正确求助?哪些是违规求助? 3332927
关于积分的说明 10258351
捐赠科研通 3048347
什么是DOI,文献DOI怎么找? 1673093
邀请新用户注册赠送积分活动 801623
科研通“疑难数据库(出版商)”最低求助积分说明 760303