抗坏血酸
光电流
生物测定
小RNA
检出限
生物传感器
化学
氧化还原
生物物理学
纳米技术
材料科学
生物化学
生物
色谱法
无机化学
光电子学
基因
遗传学
食品科学
作者
Weijing Yi,Ruili Cai,Dongfang Xiang,Yanxia Wang,Mengsi Zhang,Qinghua Ma,You‐Hong Cui,Xiu‐Wu Bian
标识
DOI:10.1016/j.bios.2019.111614
摘要
An ultrasensitive photoelectrochemical (PEC) bioassay for determination of microRNA was proposed based on an integrative photoactive heterojunction nanomaterial to provide the basis of excellent PEC responses and an efficient redox cycling amplification system to improve the detection performances. To establish the bioassay system, the biosensor was firstly modified with Bi2WO6@Bi2S3 and alkaline phosphatase (ALP). The detection solution was composed of ascorbic acid phosphate (AAP) and ferrocenecarboxylic acid (FcA), where ALP converted AAP into ascorbic acid (AA) to trigger a process of redox cycling amplification by reducing FcA+ to FcA, resulting in enhanced photocurrent responses of Bi2WO6@Bi2S3. In the presence of microRNA 21, it could trigger a hybridization chain reaction via the special designed hairpin DNA to produce a long repeated DNA sequences to inhibit ALP activity. Thus the reduced ALP activity and consequently decreased photocurrent signal could be obtained for detection of microRNA 21. As expected, this bioassay system performed the satisfactory performances for the ultrasensitive detection of microRNA 21 in the range from 1 fM to 1 nM with an experimental detection limit of 0.26 fM and acceptable practical applicability. Collectively, an efficient PEC bioassay for microRNA 21 is established and this strategy can be expanded to detect other microRNAs, even other molecules in cells.
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