Expression of main neutralization antigen VP7 of human rotavirus serotype G2 and G3 by recombinant adenoviruses

Objective To express main neutralization antigen VP7 of human rotavirus serotype G2 and G3 by recombinant adenoviruses on the basis of previous investigation of the prevalence of rotavirus in China. Methods On the basis of successfully expression of human rotavirus protein G1VP7 by recombinant adenovirus vector,the authors constructed some potent recombinant adenovirus strains encoding rotavirus G2 VP7 and G3 VP7 genes which belong to the main rotavirus isolates 97S43 and 97S48. Results Replication-defective recombinant adenoviruses expressing human rotavirus serotype G2 and G3 VP7 genes,named as rvAdG2VP7 and rvAdG3VP7 were successfully constructed. VP7 genes integrated into the viral genome were identified by PCR and Southern blot assay,and specific transcription were detected by RT-PCR in the 293 cells infected with recombinant adenoviruses. Expression of rotavirus VP7 proteins was demonstrated by Western blot assay. Conclusion The established recombinant adenoviruses expressing G2 and G3 serotype VP7s laid a significant basis for further animal experiments in the development of multivalent rotavirus vaccines against rotavirus infection.