灯1
溶酶体
蛋白酶体
黑质
α-突触核蛋白
生物
神经黑素
细胞生物学
组织蛋白酶D
神经退行性变
帕金森病
包涵体
内分泌学
内科学
生物化学
医学
疾病
酶
大肠杆菌
基因
作者
Yaping Chu,Hemraj B. Dodiya,Patrick Aebischer,C. Warren Olanow,Jeffrey H. Kordower
标识
DOI:10.1016/j.nbd.2009.05.023
摘要
We explored the relationship between ubiquitin proteasome system (UPS) and lysosomal markers and the formation of α-synuclein (α-syn) inclusions in nigral neurons in Parkinson disease (PD). Lysosome Associated Membrane Protein 1(LAMP1), Cathepsin D (CatD), and Heat Shock Protein73 (HSP73) immunoreactivity were significantly decreased within PD nigral neurons when compared to age-matched controls. This decrease was significantly greater in nigral neurons that contained α-syn inclusions. Immunoreactivity for 20S proteasome was similarly reduced in PD nigral neurons, but only in cells that contained inclusions. In aged control brains, there is staining for α-syn protein, but it is non-aggregated and there is no difference in LAMP1, CatD, HSP73 or 20S proteasome immunoreactivity between α-syn positive or negative neuromelanin-laden nigral neurons. Targeting over-expression of mutant human α-syn in the rat substantia nigra using viral vectors revealed that lysosomal and proteasomal markers were significantly decreased in the neurons that displayed α-syn-ir inclusions. These findings suggest that α-syn aggregation is a key feature associated with decline of proteasome and lysosome and support the hypothesis that cell degeneration in PD involves proteosomal and lysosomal dysfunction, impaired protein clearance, and protein accumulation and aggregation leading to cell death.
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