卵清蛋白
生物
分子生物学
雌激素受体
转染
激素反应元件
细胞生物学
基因
遗传学
免疫系统
癌症
乳腺癌
作者
Marie‐Pierre Gaub,María Elena de Bellard,I Scheuer,Pierre Chambon,Paolo Sassone‐Corsi
出处
期刊:Cell
[Elsevier]
日期:1990-12-01
卷期号:63 (6): 1267-1276
被引量:457
标识
DOI:10.1016/0092-8674(90)90422-b
摘要
The proximal region of the ovalbumin gene promoter contains a half-palindromic estrogen-responsive element (ERE) that mediates cell-specific trans-activation by the estrogen receptor (ER). We show that the ovalbumin ERE binds a ubiquitous nucleoprotein complex containing oncoproteins c-Fos and c-Jun. Mutations altering the estrogen inducibility of the promoter prevent the complex formation, which is, however, found in the presence and absence of ER and estradiol. Mutagenesis indicates that the sequence 5'-TGGGTCA-3', containing the half-palindromic ERE, is responsible for induction by phorbol esters of the ovalbumin promoter and is a target for c-fos and c-jun trans-activation. Transfection experiments reveal that c-fos, c-jun, and ER coactivate the ovalbumin promoter. Direct ER interaction with the target sequence is not required, since an ER deleted for its DNA binding domain is functional in the coactivation with c-fos and c-jun. Our data indicate a convergence of hormonal induction and activation of signal transduction pathways at the transcriptional level.
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