MAPK/ERK通路
p38丝裂原活化蛋白激酶
激酶
化学
一氧化氮
一氧化氮合酶
肿瘤坏死因子α
脂多糖
蛋白激酶A
NF-κB
信号转导
药理学
生物化学
生物
内分泌学
有机化学
作者
Kil-Nam Kim,Yeong-Jong Ko,Hye-Won Yang,Young-Min Ham,Seong Woon Roh,You‐Jin Jeon,Ginnae Ahn,Min‐Cheol Kang,Weon‐Jong Yoon,Daekyung Kim,Tatsuya Oda
标识
DOI:10.1016/j.fct.2013.03.017
摘要
We investigated the composition of essential oil from fingered citron (Citrus medica L. var. sarcodactylis) (FCEO) peels by GC–MS and its anti-inflammatory effects on lipopolysaccharide (LPS) – stimulated mouse macrophage (RAW 264.7) cells. Fifteen compounds, representing 98.97% of the essential oil, were tentatively identified; the main constituents were limonene (52.44%) and γ-terpinene (28.41%). FCEO significantly inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) by suppressing the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, respectively. Additionally, FCEO suppressed the production of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6. FCEO attenuated LPS-induced nuclear factor-κB (NF-κB) activation via inhibition of inhibitor κB-α phosphorylation. Furthermore, FCEO blocked activation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) but not that of p38 mitogen-activated protein kinase. These results indicate that FCEO inhibits LPS-stimulated inflammation by blocking the NF-κB, JNK, and ERK pathways in macrophages, and demonstrate that FCEO possesses anti-inflammatory properties.
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