Effects of proteose‐peptone fractions from yak milk on lipoprotein lipase lipolysis

The lipoprotein lipase (LPL) was purified almost 3800‐fold from yak skimmilk by Heparin‐Sepharose CL‐6B column chromatography; nonhydrophobic fraction of proteose‐peptone (NHFPP) and hydrophobic fraction of proteose‐peptone (HFPP) from yak milk whey were separated by Phenyl‐Sepharose‐6FF column chromatography. The HFPP was subjected to hydroxyapatite chromatography, and two fractions were collected: one fraction did not absorb onto the calcium phosphate matrix (HA1); the other fraction contained all the protein components of HFPP (HA2). The effects of the proteose‐peptone fractions on lipoprotein lipase lipolysis were studied. The results of experiments showed that NHFPP and HA1 enhanced the LPL lipolysis; in contrast, the HFPP and HA2 inhibited LPL lipolysis.