Catalyzing Carbohydrate Cleavage: Glycosidases and Their Mechanisms

Glycoside hydrolases, “glycosidases”, catalyze carbohydrate catabolism, remodeling, and signaling by accelerating glycosidic-bond cleavage by more than 17 orders of magnitude. Distributed across every kingdom of life and grouped into over 180 sequence-defined families, these enzymes exhibit exceptional diversity in fold, mechanism, and physiological function, and many also catalyze transglycosylation or phosphorolysis. The classical Koshland paradigms─stereochemical inversion, enzymatic nucleophile-assisted retention, and substrate-assisted retention─are analyzed with an emphasis on the conformational itineraries and oxocarbenium ion-like transition states revealed by kinetic isotope effects, linear free-energy relationships, and high-resolution three-dimensional structures. Attention then turns to noncanonical enzymes that employ NAD+-dependent redox hydrolysis or other cleavage mechanisms. Mechanistic insights have inspired the development of engineered glycosidase-derived catalysts for programmed bond construction as well as mechanism-based inhibitors, transition-state analogues, and activity-based probes that are driving advances in chemical biology, biotechnology, and drug discovery.