肝星状细胞
肝硬化
肝移植
纤维化
肝细胞癌
肝纤维化
慢性肝病
癌症研究
生物
肝纤维化
肝病
表型
医学
病理
移植
内科学
基因
遗传学
作者
Shan Yu,Matthew Ericson,Andrea Fanjul,Derek M. Erion,Maria D. Paraskevopoulou,Erin N. Smith,Banumathi K. Cole,Ryan E. Feaver,Corine Holub,Narender Gavva,Shane R. Horman,Jie Huang
标识
DOI:10.1021/acschembio.2c00006
摘要
Liver fibrosis progression in chronic liver disease leads to cirrhosis, liver failure, or hepatocellular carcinoma and often ends in liver transplantation. Even with an increased understanding of liver fibrogenesis and many attempts to generate therapeutics specifically targeting fibrosis, there is no approved treatment for liver fibrosis. To further understand and characterize the driving mechanisms of liver fibrosis, we developed a high-throughput genome-wide CRISPR/Cas9 screening platform to identify hepatic stellate cell (HSC)-derived mediators of transforming growth factor (TGF)-β-induced liver fibrosis. The functional genomics phenotypic screening platform described here revealed the novel biology of TGF-β-induced fibrogenesis and potential drug targets for liver fibrosis.
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