运动发酵单胞菌
半胱氨酸
生物化学
化学
谷胱甘肽
木质纤维素生物量
水解物
乙醇燃料
糠醛
发酵
酶
水解
催化作用
作者
Xiongying Yan,Xia Wang,Yongqiang Yang,Zhen Wang,Haoyu Zhang,Li Yang,Qiaoning He,Mian Li,Shihui Yang
标识
DOI:10.1016/j.biortech.2022.126878
摘要
Inhibitors in lignocellulosic hydrolysates are toxic to Zymomonas mobilis and reduce its bioethanol production. This study revealed cysteine supplementation enhanced furfural tolerance in Z. mobilis with a 2-fold biomass increase. Transcriptomic study illustrated that cysteine biosynthesis pathway was down-regulated while cysteine catabolism was up-regulated with cysteine supplementation. Mutants for genes involved in cysteine metabolism were constructed, and metabolites in cysteine metabolic pathway including methionine, glutathione, NaHS, glutamate, and pyruvate were supplemented into media. Cysteine supplementation boosted glutathione synthesis or H2S release effectively in Z. mobilis leading to the reduced accumulation of reactive oxygen species (ROS) induced by furfural, while pyruvate and glutamate produced in the H2S generation pathway promoted cell growth by serving as the carbon or nitrogen source. Finally, cysteine supplementation was confirmed to enhance Z. mobilis tolerance against ethanol, acetate, and corncob hydrolysate with an enhanced ethanol productivity from 0.38 to 0.55 g-1∙L-1∙h-1.
科研通智能强力驱动
Strongly Powered by AbleSci AI