Nitrite accumulation, denitrification kinetic and microbial evolution in the partial denitrification process: The combined effects of carbon source and nitrate concentration

The combined effects of carbon source (HAc, HPr, Glu, Glu + HAc) and nitrate concentration (40, 80 mg/L labeling as R40, R80) on partial denitrification (PD) were discussed at C/N ratio of 2.5 (COD = 100, 200 mg/L). The optimal NO2−-N and NTR reached to 67.03 mg/L, 99.14% in HAc-R80 system, and denitrification kinetics revealed the same conclusion, corresponding to higher COD utilization rate (CUR: 58.46 mgCOD/(gVSS·h)), nitrate reduction rate (NaRR: 29.94 mgN/(gVSS·h)) and nitrite accumulation rate (NiAR: 29.68 mgN/(gVSS·h)). The preference order was HAc > HPr > Glu + HAc > Glu in both R40 and R80 systems due to different metabolic pathways, however, the NO2−-N accumulation and kinetic parameters of R80 group were dramatically higher than those in R40 for the same carbon source. The R80 group facilitated more concentrated biodiversity (607–808 OTUs) with Terrimonas and norank_f_Saprospiraceae responsible for high NO2−-N accumulation in HAc and HPr served systems, while norank_f_norank_o_Saccharimonadales and OLB13 dominated the Glu containing systems.