单元格排序
间充质干细胞
祖细胞
细胞生物学
背景(考古学)
脂肪生成
分离(微生物学)
生物
间质细胞
细胞
计算生物学
干细胞
生物信息学
癌症研究
遗传学
古生物学
作者
Marcela Low,Christine Eisner,Fabio M.V. Rossi
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 179-189
被引量:17
标识
DOI:10.1007/978-1-4939-6771-1_9
摘要
Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.
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