随机六聚体
多克隆抗体
肽
单克隆抗体
化学
抗体
组氨酸
亲和层析
氨基酸
选择性
生物化学
分子生物学
色谱法
生物
免疫学
催化作用
酶
作者
Hong Yang,Patrick V. Gurgel,Ruben G. Carbonell
出处
期刊:Journal of Peptide Research
[Wiley]
日期:2005-12-01
卷期号:66 (s1): 120-137
被引量:110
标识
DOI:10.1111/j.1747-0285.2006.00342.x
摘要
Abstract: A family of linear hexapeptides composed of histidine on the N‐terminus followed by aromatic amino acid(s) and positively charged amino acid(s) has been identified through a three‐step screening of a synthetic solid phase library. These peptides were able to recognize human immunoglobulin G (HIgG) through its Fc region, and their selectivity to Fc is comparable to Protein A. This is the first known report of short peptides that are able to bind HIgG by recognizing its Fc portion. One of the ligands from the identified family, HWRGWV, was examined for its ability to isolate HIgG from complex mixtures. It was found that HWRGWV possessed the potential to purify HIgG from complete mammalian cell culture medium containing 10% fetal calf serum with purity comparable to commercially available resins, but using milder elution conditions. HWRGWV bound all HIgG subclasses and IgGs from bovine, mouse, goat, and rabbit. The broad affinity spectrum as well as its Fc recognition ability may be useful in capturing and detecting both polyclonal and monoclonal antibodies.
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