Interactions of Dialdehyde Bletilla striata Polysaccharide With Fibrin and Their Acceleration on Wound Healing

ABSTRACT Oxidized polysaccharides have garnered significant attention as fillers for the development of reinforced composites, particularly in the biomedical field. In this study, we oxidized Bletilla striata polysaccharide (BSP) using sodium periodate to produce dialdehyde Bletilla striata polysaccharide (A‐OBSP). We then explored the potential of incorporating A‐OBSP into fibrinogen to create composite hydrogels. The inclusion of A‐OBSP notably altered the initial fibrin fibrillogenesis, resulting in more compact and organized fibrin networks. The storage moduli of the composite hydrogels were significantly higher than those of pure fibrin hydrogels. Furthermore, the presence of A‐OBSP enhanced water swelling and slowed the degradation of the hydrogels in phosphate‐buffered saline. The A‐OBSP‐enhanced composite hydrogels exhibited remarkable antibacterial, antioxidant, and anti‐inflammatory properties. In a murine wound healing model, the hydrogel containing 30 mg/mL A‐OBSP demonstrated superior wound healing efficacy compared to the pure fibrin group, as evidenced by the growth of hair follicles and capillaries in the granulation tissue, with a 100% healing rate by day 21. Additionally, the fibrin/A‐OBSP composite hydrogels showed significantly better therapeutic outcomes for wound healing than OBSP (TEMPO oxidation) and BSP, as reported in our previous studies. Therefore, fibrin/A‐OBSP composites hold substantial promise as wound healing dressings.