免疫系统
生物
免疫学
髓样
流式细胞术
细胞毒性T细胞
免疫分型
自然杀伤细胞
外周血单个核细胞
髓源性抑制细胞
T细胞
抑制器
体外
遗传学
癌症
作者
Yinchun Chen,Haimei Deng,Ruiqing Zhou,Xiaotao Jiang,Huijuan Wang,Songqing Xin,Wenjian Mo,Shunqing Wang,Yufeng Liu
标识
DOI:10.1093/jleuko/qiae138
摘要
Abstract Secondary hemophagocytic lymphohistiocytosis (sHLH) is a hyperinflammatory syndrome characterized by immune disorders. It is imperative to elucidate the immunophenotypic panorama and the interactions among these cells in patients. Human peripheral blood mononuclear cells were collected from healthy donors and sHLH patients and tested using multicolor flow cytometry. We used FlowSOM to explore and visualize the immunophenotypic characteristics of sHLH. By demonstrating the phenotypes of immune cells, we discovered that sHLH patients had significantly higher levels of CD56+ monocytes, higher levels of myeloid-derived suppressor cells, low-density neutrophil-to-T cell ratio, and higher heterogeneous T cell activation than healthy donors. However, natural killer cell cytotoxicity and function were impaired. We then assessed the correlations among 30 immune cell types and evaluated metabolic analysis. Our findings demonstrated polymorphonuclear myeloid-derived suppressor cells, CD56+ monocytes, and neutrophil-to-T cell ratio were elevated abnormally in sHLH patients, which may indicate an association with immune overactivation and inflammatory response. We are expected to confirm that they are involved in the occurrence of the disease through further in-depth research.
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