Spinning arrayed optofluidic imaging for large-scale single-cell image-based analysis (Conference Presentation)

We report an arrayed optofluidic imaging platform that allows multiplexed image-based cell assay at ultrahigh imaging throughput. The assay platform, consisting of 96 fluidic sample chambers arranged in a circular symmetry, operates in a reconfigurable spinning motion synchronized with an ultrafast laser-scanning microscope (a line-scan rate >10 MHz). Based on a stable through-focus spinning mechanism, the assay platform allows ultralarge field-of-view imaging (18.8 cm2, >160 Gpixels) with high image fidelity and subcellular resolution (in both the bright-field and quantitative phase image contrasts). We further validated that the continuous spinning operation has minimal impact on the cell morphology and viability.