细胞器
显微镜
光漂白后的荧光恢复
光漂白
荧光显微镜
相衬显微术
活体细胞成像
显微镜
生物物理学
生物系统
计算机科学
纳米技术
化学
材料科学
荧光
生物
细胞
物理
细胞生物学
光学
生物化学
作者
Jingde Fang,Hao Zhang,Yang Pan,Zachary J. Smith,Kaiqin Chu
出处
期刊:ACS Photonics
[American Chemical Society]
日期:2023-03-17
被引量:2
标识
DOI:10.1021/acsphotonics.2c01782
摘要
Organelles are highly dynamic and fulfill their function by constant motion and cooperation with each other. Current methods rely on fluorescence, leading to short observation time (via photobleaching) and experimental complexity (via multiple labeling). While label-free microscopes promise a paradigm change in this regard, the spatiotemporal resolutions and specificity are still insufficient to study organelle interactions. Using mitochondria and lysosome as examples, we demonstrate that our organelle-specific phase contrast microscopy (OS-PCM) can achieve automatic analysis of dynamic metrics of multiple organelles as well as their interactions from unlabeled cells for the first time. Compared to fluorescence-based methods, our method is gentle and holds great promise for label-free visualization and analysis of pan-organelle dynamics and interactions, with minimum perturbation to the cell.
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