DNA
微流控
Spike(软件开发)
寡核苷酸
微尺度化学
适体
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
病毒学
计算生物学
生物
病毒包膜
病毒
2019年冠状病毒病(COVID-19)
纳米技术
材料科学
计算机科学
传染病(医学专业)
分子生物学
遗传学
数学
软件工程
病理
医学
数学教育
疾病
作者
Yihao Huang,Qiuyue Wu,Jialü Zhang,Yuqian Zhang,Shiyun Cen,Chaoyong Yang,Yanling Song
出处
期刊:ACS Nano
[American Chemical Society]
日期:2023-10-30
卷期号:17 (21): 21973-21983
标识
DOI:10.1021/acsnano.3c08400
摘要
Health diagnostic tools for community safety and environmental monitoring require selective and quantitatively accurate active viral load assessment. Herein, we report a microfluidic enrichment strategy to separate intact SARS-CoV-2 particles by AND logic gate with inputs of cholesterol oligonucleotides for the envelope and aptamers for the spike viral proteins. Considering the unequal quantity of endogenous spikes and lipid membranes on SARS-CoV-2, a dual-domain binding strategy, with two aptamers targeting different spike domains, was applied to balance the spike-envelope stoichiometric ratio. By balancing the stoichiometric with DNA computation and promoting microscale mass transfer of the herringbone chip, the developed strategy enabled high sensitivity detection of pseudotyped SARS-CoV-2 with a limit of detection as low as 37 active virions/μL while distinguishing it from inactive counterparts, other nontarget viruses, and free spike protein. Moreover, the captured viral particles can be released through DNase I treatment with up to 90% efficiency, which is fully compatible with virus culture and sequencing. Overall, the developed strategy not only identified SARS-CoV-2-infected patients (n = 14) with 100% identification from healthy donors (n = 8) but also provided a fresh perspective on the regulation of stoichiometric ratio to achieve a more biologically relevant DNA computation.
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