Multiactivity of Nanozyme Liposome Regulates the Homeostasis of Biochemical Microenvironment

In living systems, cascade reactions involving multiple enzymes, critical for regulating the biochemical microenvironment's homeostasis, are essential to transplantation remodeling. However, for ready-to-use purposes, designing and constructing therapeutic liposomes that replicate natural cell functions during the initial transplantation phase remains a significant challenge in synthetic biology for tissue engineering. Herein, we developed a biomimetic liposome with multienzymatic nanozyme activity to regulate proinflammatory factors induced by exogenous implants. The liposomes were fabricated through the self-assembly of artificial membranes on CeO₂ nanoparticles (NPs). These membranes, featuring a negatively charged surface analogous to endothelial cell (EC) membranes, effectively attenuate blood component adhesion and aggregation. The CeO₂ nanozyme not only hydrolyzes adenosine triphosphate/adenosine diphosphate (ATP/ADP) to adenosine monophosphate (AMP), reducing subsequent platelet aggregation, but also regulates biochemical microenvironment homeostasis, thereby promoting tissue regeneration. This work advances the development of cell-like entities capable of modulating signaling communication between living and abiotic interfaces.