清脆的
生物
基因组工程
基因组编辑
合成生物学
计算生物学
Cas9
基因组
转化式学习
细菌基因组大小
生物技术
遗传学
基因
心理学
教育学
作者
Daniel C. Volke,Enrico Orsi,Pablo I. Nikel
标识
DOI:10.1016/j.mib.2023.102353
摘要
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated proteins (Cas) technologies brought a transformative change in the way bacterial genomes are edited, and a plethora of studies contributed to developing multiple tools based on these approaches. Prokaryotic biotechnology benefited from the implementation of such genome engineering strategies, with an increasing number of non-model bacterial species becoming genetically tractable. In this review, we summarize the recent trends in engineering non-model microbes using CRISPR–Cas technologies, discussing their potential in supporting cell factory design towards biotechnological applications. These efforts include, among other examples, genome modifications as well as tunable transcriptional regulation (both positive and negative). Moreover, we examine how CRISPR–Cas toolkits for engineering non-model organisms enabled the exploitation of emergent biotechnological processes (e.g. native and synthetic assimilation of on-carbon substrates). Finally, we discuss our slant on the future of bacterial genome engineering for domesticating non-model organisms in light of the most recent advances in the ever-expanding CRISPR–Cas field.
科研通智能强力驱动
Strongly Powered by AbleSci AI