外显子
表皮(动物学)
RNA剪接
选择性拼接
基因亚型
细胞生物学
生物
RNA结合蛋白
信使核糖核酸
细胞分化
遗传学
基因
核糖核酸
解剖
作者
Shota Takashima,Wujianan Sun,A. Otten,Pengfei Cai,Shaohong Peng,Elton Tong,Jennifer M. Bui,Mingxing Mai,Oyumergen Amarbayar,Baohua Cheng,Rowen Jane Odango,Z. Li Zengmin Li,Kun Qu,Bryan K. Sun
出处
期刊:Cell Reports
[Elsevier]
日期:2024-03-01
卷期号:43 (3): 113814-113814
标识
DOI:10.1016/j.celrep.2024.113814
摘要
Summary
Alternative splicing (AS) of messenger RNAs occurs in ∼95% of multi-exon human genes and generates diverse RNA and protein isoforms. We investigated AS events associated with human epidermal differentiation, a process crucial for skin function. We identified 6,413 AS events, primarily involving cassette exons. We also predicted 34 RNA-binding proteins (RBPs) regulating epidermal AS, including 19 previously undescribed candidate regulators. From these results, we identified FUS as an RBP that regulates the balance between keratinocyte proliferation and differentiation. Additionally, we characterized the function of a cassette exon AS event in MAP3K7, which encodes a kinase involved in cell signaling. We found that a switch from the short to long isoform of MAP3K7, triggered during differentiation, enforces the demarcation between proliferating basal progenitors and overlying differentiated strata. Our findings indicate that AS occurs extensively in the human epidermis and has critical roles in skin homeostasis.
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