Distinct responses of Chlorella vulgaris upon combined exposure to microplastics and bivalent zinc

Microplastics (MPs) and heavy metals are ubiquitous pollutants in the aquatic environment. In this study, the sorption behavior of two typical MPs (PVC and PE) to bivalent zinc ions (Zn(II)) and their combined toxic effects on Chlorella vulgaris were systemically studied. The growth inhibition rate, the activities of photosynthesis and antioxidant enzymes (SOD and CAT), the cell membrane integrity and the cell apoptosis rate were employed to evaluate the toxicity. Our result showed that PVC and PE have different adsorption capacities for Zn(II), and the combined exposure to Zn(II) and MPs had distinct patterns on the inhibition of the cell growth and induction of oxidative stress. Under our experimental concentrations, PE and Zn(II) showed a synergistic effect, while PVC and Zn(II) exhibited an antagonistic effect. Finally, an action mechanism was proposed to explain the experimental phenomena. This study demonstrated that flow cytometry can be a powerful tool to study the toxic effect of MP composites, and MPs can not only allow a free ride for the water contaminants, but also remarkably alter their toxic effects on phytoplankton. These effects deserve further consideration during evaluation of ecological risks of MPs in the water environment.