微泡
细胞外小泡
胞外囊泡
纳米技术
标准化
药物输送
计算机科学
生化工程
生物
细胞生物学
材料科学
工程类
生物化学
基因
操作系统
小RNA
作者
Anjana Jeyaram,Steven M. Jay
出处
期刊:Aaps Journal
[Springer Nature]
日期:2017-11-27
卷期号:20 (1)
被引量:276
标识
DOI:10.1208/s12248-017-0160-y
摘要
Recently, extracellular vesicles (EVs)-including exosomes, microvesicles, and others-have attracted interest as cell-derived biotherapeutics and drug delivery vehicles for a variety of applications. This interest stems from favorable properties of EVs, including their status as mediators of cell-cell communication via transfer of biological cargo and their reported ability to cross biological barriers that impede many delivery systems. However, there are many challenges to translation and widespread application of EV-based therapeutics. One such challenge that has yet to be extensively studied involves EV preservation and storage, which must be addressed to enable use of therapeutic EVs beyond resource-intensive settings. Studies to date suggest that the most promising mode of storage is - 80°C; however, understanding of storage-mediated effects is still limited. Additionally, the effects of storage appear to vary with sample source. The lack of knowledge about and standardization of EV storage may ultimately hinder widespread clinical translation. This mini-review reports current knowledge in the field of EV preservation and storage stability and highlights future directions in the area that could be critical to eventual development of EV therapies.
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