粘附
生物材料
材料科学
蛋白质吸附
生物相容性
硅酮
细胞粘附
吸附
表面粗糙度
硅橡胶
形态学(生物学)
表面能
复合材料
化学工程
化学
纳米技术
冶金
聚合物
有机化学
生物
工程类
遗传学
作者
Nadim J. Hallab,K.J. Bundy,Kim C. O'Connor,Rhett J. Clark,R. L. Moses
出处
期刊:PubMed
日期:1995-01-01
卷期号:5 (3): 209-31
被引量:30
摘要
Adhesion of cells to a biomaterial surface can be a major factor mediating its biocompatibility. In this investigation, jet impingement techniques were used to quantify strength of cellular adhesion to various material surfaces. The metals tested: HS25 (a cobalt-based alloy similar to F75), 316L stainless steel, Ti-6Al-4V, and commercially pure tantalum, exhibited nearly a fivefold increase in adhesion strength above that characteristic of the polymeric materials tested (PTFE, silicone rubber, and HDPE). The present study examines physical and biological factors that might influence fibroblast adhesion to the biomaterial surface. The relation between surface charge and cellular adhesion was investigated in a controlled manner by measuring adhesion strength over a range of charge densities. The cells showed charge and electrical potential-dependent adhesion maxima, suggesting that surface alloying for optimum adherence may be possible. In a preliminary series of experiments adsorbed serum protein layers on a series of materials of differing adherence were investigated using gel electrophoresis to assess protein composition. Analysis of adsorbed proteins revealed little difference in relative abundance or total adsorption quantity. SEM micrographs of cells on Ti-6Al-4V and silicone rubber (high and low adhesion materials, respectively) demonstrated differences in cell morphology and cell density.
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