甾醇调节元件结合蛋白
安普克
PI3K/AKT/mTOR通路
化学
P70-S6激酶1
蛋白激酶B
丁酸盐
丁酸钠
信号转导
蛋白激酶A
生物化学
细胞生物学
生物
磷酸化
转录因子
基因
发酵
作者
Ji Cheng,Yufei Zhang,Yakun Ge,Wen Li,Yu Cao,Yue Qu,Bo Liu,Yunlong Guo,Shoupeng Fu,Juxiong Liu
出处
期刊:Life Sciences
[Elsevier]
日期:2020-10-01
卷期号:259: 118375-118375
被引量:22
标识
DOI:10.1016/j.lfs.2020.118375
摘要
Short-chain fatty acids were reported to be the precursors of milk fat and can stimulate the de novo synthesis of fatty acids in bovine mammary epithelial cells (bMECs). However, the mechanism has not been elucidated. The purpose of this study was to investigate the effects of sodium butyrate (NaB) on milk fat synthesis in bMECs and explore its potential mechanism.Bovine mammary epithelial cells (bMECs) were isolated for subsequent experimental uses. BODIPY staining and triglyceride kit were used to detect the milk fat synthesis in bMECs. Western blotting and RT-PCR assays were performed to detect the expression of related genes in bMECs. Immunoprecipitation was used to detect the acetylation of SREBP1 in bMECs.The results showed that NaB significantly promoted milk fat synthesis, promoted the activity of mechanistic target of rapamycin (mTOR) and S6 kinase (S6K), inhibited the activity of AMP-activated protein kinase (AMPK), and promoted the gene expression of G protein-coupled receptor 41 (GPR41). Knockdown of GPR41 and sterol regulatory element binding protein 1 (SREBP1) and overexpression of sirtuin1 (SIRT1), mTOR inhibitor (rapamycin), and AMPK activator (AICIR) eliminated these effects. These results indicated that NaB increased the nuclear translocation of SREBP1 via the GPR41/AMPK/mTOR/S6K signalling pathway, promoted the acetylation of mature SREBP1a via GPR41/AMPK/SIRT1, and then promoted milk fat synthesis.Taken together, these results demonstrated that NaB increased nuclear translocation and acetylation of SREBP1 to promote milk fat synthesis by activating GPR41 and its downstream signalling pathways.
科研通智能强力驱动
Strongly Powered by AbleSci AI