栅栏
光学
显微镜
衍射光栅
干扰(通信)
材料科学
数字微镜装置
衍射
激光器
激发
光电子学
物理
计算机科学
计算机网络
量子力学
频道(广播)
作者
Meiqi Li,Yaning Li,Wenhui Liu,Amit Lal,Shan Jiang,Dayong Jin,Houpu Yang,Shu Wang,Karl Zhanghao,Peng Xi
摘要
Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple laser beams, which are diffracted from a grating. In SIM, 2D imaging requires 9 patterns and 3D imaging requires 15 patterns. Compared to mechanical movement of gratings, opti-electro devices provide rapid switch of the excitation patterns, in which Digital Micro-mirror Device (DMD) is most common in industry. Here we model DMD as the blazed grating and report a fast and cost-efficient SIM. Our home-built DMD-based laser interference structured illumination microscopy (DMD-ISIM) system reveals the nuclear pore complex and microtubule in mammalian cells with doubled spatial resolution. We further proposed multi-color DMD-ISIM system with simulation, which could potentially exploit the full power of DMD-ISIM.
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